Recombinant Murine IGF-I
Catalog Number : 250-19
The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant murine IGF-I is a 7.6 kDa globular protein containing amino acids including 3 intra-molecular disulfide bonds.
Above 98% as determined by SDS-PAGE Analysis.
The ED50 was determined a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105<
GPETLCGAE LVDALQFVC GPRGFYFNK PTGYGSSIR RAPQTGIVD ECCFRSCDL RRLEMYCAP LKPTKAA
|Research Interest:||Angiogenesis, Bone Biology, Proliferation and Cell Cycle, Metabolic Regulation and Diabetes|
|Protein Cross Reactivity:||Rat|