Recombinant Human HB-EGF
Catalog Number : 100-47
HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.
Above 95% as determined by SDS-PAGE Analysis.
The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
DLQEADLDL LRVTLSSKP QALATPNKE EHGKRKKKG KGLGKKRDP CLRKYKDFC IHGECKYVK ELRAPSCIC HPGYHGERC HGLSL
|Research Interest:||Apoptosis, Angiogenesis, Proliferation and Cell Cycle|
|Cell Type:||Macrophage / Monocyte|
|Protein Cross Reactivity:||NULL|