Catalog Number : 500-M130
Produced in BALB/c mice using highly pure recombinant Rat IL-10 as the immunizing antigen. This antibody was purified from tissue culture by Protein A affinity chromatography.
|Application:||ELISA, Western Blot|
Anti-Rat IL-10 (BioGems Catalog #400-19)
In a sandwich ELISA (assuming 100μl/well), a concentration of 8.0-16.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with BioGems' Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (62-010BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.