Anti-Murine IL-2

Grouped product items
SizePrice Qty
500μg
$150.00
Catalog Number : 500-M127

description

Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-2 as the immunizing antigen. This IgG2Aκ antibody was purified from cell culture by Protein A affinity chromatography.

Additional Information

Application:ELISA, Western Blot
Immunogen
Anti-Murine IL-2 (BioGems Catalog #212-12)
Clone:
Monoclonal Rat
Format:
purified
Applications:
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 32 pg/ml of recombinant murine IL-2 when used with BioGems' biotinylated antigen affinity purified anti-murine IL-2 (61-002BT) as the detection antibody at a concentration of at least 1.0-2.0 µg/ml.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Reactivity:
Mouse
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