Anti-Murine IL-10

Grouped product items
SizePrice Qty
500μg
$150.00
Catalog Number : 500-M128

description

Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-10 as the immunizing antigen. This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.

Additional Information

Application:ELISA, Western Blot, Neutralization
Immunogen
Anti-Murine IL-10 (BioGems Catalog #210-10)
Clone:
Monoclonal Rat
Format:
purified
Applications:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with BioGems' biotinylated antigen affinity purified anti-murine IL-10 (61-010BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Western Blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Reactivity:
Mouse
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