Catalog Number : 500-M91
Produced in BALB/c mice using highly pure (>98%) recombinant human Resistin as the immunizing antigen. This IgG2bκ antibody was purified from cell culture by Protein A affinity chromatography.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human Resistin (BioGems Catalog #450-19)
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 μg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with BioGems' biotinylated antigen affinity purified anti-human Resistin (60-289BT) as the detection antibody at a concentration of at least 0.5-1.0 µg/ml.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.