Anti-Human RANTES

Grouped product items
SizePrice Qty
500μg
$150.00
Catalog Number : 500-M75

description

Produced in BALB/c mice using highly pure (>98%) human RANTES as the immunizing antigen. This IgG2bK antibody was purified from ascites fluid by Protein A affinity chromatography.

Additional Information

Application:ELISA, Western Blot, Neutralization, Immunohistochemistry
Immunogen
Anti-Human RANTES (BioGems Catalog #300-06)
Clone:
Monoclonal Mouse
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.

*Additional Immunostaining data available. Please contact Tech Support for information.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with BioGems' biotinylated antigen affinity purified anti-human RANTES (60-285BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Reactivity:
Human
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