Catalog Number : 500-M75
Produced in BALB/c mice using highly pure (>98%) human RANTES as the immunizing antigen. This IgG2bK antibody was purified from ascites fluid by Protein A affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human RANTES (BioGems Catalog #300-06)
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
*Additional Immunostaining data available. Please contact Tech Support for information.
To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with BioGems' biotinylated antigen affinity purified anti-human RANTES (60-285BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.