Anti-Human MIP-3β

Grouped product items
SizePrice Qty
500μg
$150.00
Catalog Number : 500-M29

description

Produced in BALB/c mice using highly pure recombinant Human MIP-3β as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Additional Information

Application:ELISA, Western Blot, Immunohistochemistry
Immunogen
Anti-Human MIP-3β (BioGems Catalog #300-29B)
Clone:
Monoclonal Mouse
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained PBMC.  The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. 
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant Human MIP-3β when used with BioGems' biotinylated antigen affinity purified anti-Human MIP-3β (60-228BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Western Blot:
To detect Human MIP-3β by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3β is 2.0-4.0 ng/lane, under reducing conditions.
Reactivity:
Human
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