Catalog Number : 500-M29
Produced in BALB/c mice using highly pure recombinant Human MIP-3β as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human MIP-3β (BioGems Catalog #300-29B)
This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant Human MIP-3β when used with BioGems' biotinylated antigen affinity purified anti-Human MIP-3β (60-228BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect Human MIP-3β by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3β is 2.0-4.0 ng/lane, under reducing conditions.