Catalog Number : 500-M28
Produced in mice using highly pure recombinant human MIP-3α as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
|Application:||ELISA, Western Blot|
Anti-Human MIP-3α (BioGems Catalog #300-29A)
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3α when used with BioGems' biotinylated antigen affinity purified anti-Human MIP-3α (60-227BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
To detect Human MIP-3α by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3α is 0.25-0.50 ng/lane, under non-reducing conditions.