Catalog Number : 500-M74
Produced in BALB/c mice using highly pure recombinant human MIP-1α as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human MIP-1α (BioGems Catalog #300-08)
To yield one-half maximal inhibition [ND50] of the biological activity of Human MIP-1α (100 ng/ml), a concentration of 5.0-10.0 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human MIP-1α when used with BioGems' biotinylated antigen affinity purified anti-human MIP-1α (60-224BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
To detect Human MIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions.