Catalog Number : 500-M41
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human MDC as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
|Application:||ELISA, Western Blot|
Anti-Human MDC (BioGems Catalog #300-36)
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant human MDC when used with BioGems' biotinylated antigen affinity purified anti-human MDC (60-216BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 0.25-0.50 ng/lane, under non-reducing conditions.