Catalog Number : 500-M70
Produced in BALB/c mice using highly pure (>98%) recombinant human MCP-4 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human MCP-4 (BioGems Catalog #300-24)
To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-4 (100 ng/ml), a concentration of 0.8-1.0 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-4 when used with BioGems' biotinylated antigen affinity purified anti-human MCP-4 (60-215BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.