Catalog Number : 500-M73
Produced in C3H/HeJ mice using highly pure (>98%) recombinant human MCP-3 as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human MCP-3 (BioGems Catalog #300-17)
To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-3(100 ng/ml), a concentration of 1.0-3.0 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant human MCP-3 when used with BioGems' biotinylated antigen affinity purified anti-human MCP-3 (60-214BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.