Anti-Human Leptin

Grouped product items
SizePrice Qty
500μg
$150.00
Catalog Number : 500-M27

description

Produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Additional Information

Application:ELISA, Western Blot
Immunogen
Anti-Human Leptin (BioGems Catalog #300-27)
Clone:
Monoclonal Mouse
Format:
purified
Applications:
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant human Leptin when used with BioGems' biotinylated antigen affinity purified anti-human Leptin (60-204BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Western Blot:
To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, under non-reducing conditions.
Reactivity:
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