Catalog Number : 500-M27
Produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
|Application:||ELISA, Western Blot|
Anti-Human Leptin (BioGems Catalog #300-27)
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant human Leptin when used with BioGems' biotinylated antigen affinity purified anti-human Leptin (60-204BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, under non-reducing conditions.