Catalog Number : 500-M43
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human LEC as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein G affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human LEC (BioGems Catalog #300-44)
To yield one-half maximal inhibition [ND50] of the biological activity of Human LEC (100 ng/ml), a concentration of 1.0-5.0 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human LEC when used with BioGems' biotinylated antigen affinity purified anti-human LEC (60-203BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.