Anti-Human IL-15

Grouped product items
SizePrice Qty
500μg
$150.00
Catalog Number : 500-M15

description

Produced in BALB/c x C57BL/6 F1 mice using highly pure (>98%) recombinant human IL-15 as the immunizing antigen. This IgG1K antibody was purified from ammonium sulfate precipitation followed by ion exchange chromatography.

Additional Information

Application:ELISA, Western Blot
Immunogen
Anti-Human IL-15 (BioGems Catalog #200-15)
Clone:
Monoclonal Mouse
Format:
purified
Applications:
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 250 pg/ml of recombinant human IL-15 when used with BioGems' biotinylated antigen affinity purified anti-human IL-15 (60-015BT) as the detection antibody at a concentration of approximately 2.0-4.0 µg/ml.
Western Blot:
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 µg/ml (non-reducing) or at a concentration of 2.0-4.0 µg/ml (reducing). Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Reactivity:
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