Catalog Number : 500-M11
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-11 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human IL-11 (BioGems Catalog #200-11)
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 μg/ml overnight at 4˚C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.
Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
In a sandwich ELISA (assuming 100µl/well), a concentration of 8.0-9.0 µg/ml of this antibody will detect recombinant human IL-11 when used with BioGems' biotinylated antigen affinity purified anti-human IL-11 (60-011BT) as the detection antibody at a concentration of approximately 2.0-4.0 µg/ml.
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.