Catalog Number : 500-M31
Produced in BALB/c mice using highly pure (>98%) recombinant human Eotaxin-2 as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by antigen affinity chromatography.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human Eotaxin-2 (BioGems Catalog #300-33)
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with BioGems' biotinylated antigen affinity purified anti-human Eotaxin-2 (60-123BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.