Catalog Number : 500-M121
Produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen. This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human BMP-4 (BioGems Catalog #120-05)
This antibody stained HepG2 cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.
To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5.0 ng/ml), a concentration of 0.4-0.6 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human BMP-4 when used in conjunction with compatible secondary reagents.
To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.