Biotinylated Anti-Rat GM-CSF
Catalog Number : 500-P225bt
Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Anti-Rat GM-CSF specific antibody was purified by affinity chromatography and then biotinylated.
|Application:||ELISA, Western Blot|
Biotinylated Anti-Rat GM-CSF (BioGems Catalog #400-23)
To detect Rat GM-CSF by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GM-CSF.
To detect Rat GM-CSF by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti- Rat GM-CSF (62-058P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GM-CSF.
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat GM-CSF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.