Biotinylated Anti-Rat EGF
Catalog Number : 500-P277Bt
description
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Anti-Rat EGF specific antibody was purified by affinity chromatography and then biotinylated.
Additional Information
| Application: | ELISA, Western Blot |
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Immunogen
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Biotinylated Anti-Rat EGF (BioGems Catalog #400-25)
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Clone:
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NULL
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Format:
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Biotin
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Applications:
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To detect Rat EGF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat EGF.
To detect Rat EGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Rat EGF (62-053P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat EGF.
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Reactivity:
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Rat
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