Biotinylated Anti-Murine IL-6
Catalog Number : 500-P56bt
Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IL-6. Anti-Murine IL-6 specific antibody was purified by affinity chromatography and then biotinylated.
|Application:||ELISA, Western Blot|
Biotinylated Anti-Murine IL-6 (BioGems Catalog #216-16)
To detect Murine IL-6 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-6.
To detect Murine IL-6 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Murine IL-6 (61-006P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-6.
To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.