Biotinylated Anti-Murine B7-1

Grouped product items
SizePrice Qty
25μg
$195.00
50μg
$250.00
1mg
$3,400.00
Catalog Number : 500-P128bt

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mB7-1. Anti-Murine B7-1 specific antibody was purified by affinity chromatography and then biotinylated.

Additional Information

Application:ELISA, Western Blot
Immunogen
Biotinylated Anti-Murine B7-1 (BioGems Catalog #)
Clone:
Polyclonal Rabbit
Format:
Biotin
Applications:
Direct Detection:
To detect mB7-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mB7-1.
Sandwich ELISA:
To detect mB7-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Murine B7-1 (61-401P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mB7-1.
Western Blot:
To detect mB7-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mB7-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Mouse
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