Biotinylated Anti-Human Persephin
Catalog Number : 500-P138bt
description
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPersephin. Anti-Human Persephin specific antibody was purified by affinity chromatography and then biotinylated.
Additional Information
| Application: | ELISA, Western Blot |
|---|---|
Immunogen | Biotinylated Anti-Human Persephin (BioGems Catalog #450-12) |
Clone: | Polyclonal Rabbit |
Format: | Biotin |
Applications: | To detect hPersephin by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPersephin. To detect hPersephin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human Persephin (60-271P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPersephin. To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity: | Human |