Biotinylated Anti-Human PAI-1

Grouped product items
SizePrice Qty
25μg
$195.00
50μg
$250.00
1mg
$3,400.00
Catalog Number : 500-P260bt

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1 (human Plasminogen Activator Inhibitor-1). Anti-Human PAI-1 specific antibody was purified by affinity chromatography and then biotinylated.

Additional Information

Application:ELISA, Western Blot
Immunogen
Biotinylated Anti-Human PAI-1 (BioGems Catalog #140-04)
Clone:
Polyclonal Rabbit
Format:
Biotin
Applications:
Direct Detection:
To detect hPAI-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPAI-1.
Sandwich ELISA:
To detect hPAI-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human PAI-1 (60-263P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPAI-1.
Western Blot:
To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Human
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