Biotinylated Anti-Human IL-21

Grouped product items
Size Price Qty
25μg
$195.00
50μg
$250.00
1mg
$3,400.00
Catalog Number : 500-P191bt

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-21. Anti-Human IL-21 specific antibody was purified by affinity chromatography and then biotinylated.

Additional Information

Application: ELISA, Western Blot
Immunogen
Biotinylated Anti-Human IL-21 (BioGems Catalog #200-21)
Clone:
Polyclonal Rabbit
Format:
Biotin
Applications:
Direct Detection:
To detect hIL-21 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-21.
Sandwich ELISA:
To detect hIL-21 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human IL-21 (60-021P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-21.
Western Blot:
To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
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