Biotinylated Anti-Human IGF-BP5
Catalog Number : 500-P232bt
description
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP5. Anti-Human IGF-BP5 specific antibody was purified by affinity chromatography and then biotinylated.
Additional Information
| Application: | ELISA, Western Blot |
|---|---|
Immunogen | Biotinylated Anti-Human IGF-BP5 (BioGems Catalog #100-05) |
Clone: | Polyclonal Rabbit |
Format: | Biotin |
Applications: | To detect hIGF-BP5 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-BP5. To detect hIGF-BP5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human IGF-BP5 (60-192P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-BP5. To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity: |