Anti-Rat VEGF

Grouped product items
Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P275

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Anti-Rat VEGF specific antibody was purified by affinity chromatography employing immobilized Rat VEGF matrix.

Additional Information

Application: ELISA, Western Blot, Neutralization
Immunogen
Anti-Rat VEGF (BioGems Catalog #400-31)
Clone:
Format:
purified
Applications:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat VEGF (10 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required.
Sandwich ELISA:
To detect Rat VEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat VEGF (62-078BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.
Western Blot:
To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Rat
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