Catalog Number : 500-P185G
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat Leptin. Anti-Rat Leptin specific antibody was purified by affinity chromatography employing immobilized Rat Leptin matrix.
|Application:||ELISA, Western Blot|
Anti-Rat Leptin (BioGems Catalog #400-21)
To detect Rat Leptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat Leptin (62-063BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.
To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.