Catalog Number : 500-P274
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Anti-Rat IL-2 specific antibody was purified by affinity chromatography employing immobilized Rat IL-2 matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Rat IL-2 (BioGems Catalog #400-02)
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-2 (1.0 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required.
To detect Rat IL-2 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat IL-2 (62-002BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.