Catalog Number : 500-P74
Produced from sera of rabbits pre-immunized with highly pure recombinant Rat GRO/KC. Anti-Rat GRO/KC specific antibody was purified by affinity chromatography employing immobilized Rat GRO/KC matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Rat GRO/KC (BioGems Catalog #400-10)
To yield one-half maximal inhibition [ND50] of the biological activity of Rat GRO/KC (100 ng/ml), a concentration of 5.0-10.0 μg/ml of this antibody is required.
To detect Rat GRO/KC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat GRO/KC (62-059BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GRO/KC.
To detect Rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat GRO/KC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.