Catalog Number : 500-P131
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF. Anti-Murine VEGF specific antibody was purified by affinity chromatography employing immobilized mVEGF matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Murine VEGF (BioGems Catalog #450-32)
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5μg/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (10.00 ng/ml), a concentration of 0.08-0.12 µg/ml of this antibody is required.
To detect mVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine VEGF (61-127BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.