Anti-Murine TNF-α
Catalog Number : 500-P64
description
Produced from sera of rabbits pre-immunized with highly pure recombinant Murine TNF-α. Anti-Murine TNF-α specific antibody was purified by affinity chromatography employing immobilized Murine TNF-α matrix.
Additional Information
| Application: | ELISA, Western Blot, Neutralization, Immunohistochemistry |
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Immunogen
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Anti-Murine TNF-α (BioGems Catalog #315-01A)
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Clone:
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Polyclonal Rabbit
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Format:
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purified
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Applications:
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This antibody stained colchicine injected mouse brain (hippocampus CA1 region) tissue. The primary antibody was incubated at 1.0 μg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.
Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
To yield one-half maximal inhibition [ND50] of the biological activity of Murine TNF-α (0.5 ng/ml), a concentration of 0.04 - 0.05 µg/ml of this antibody is required.
To detect Murine TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine TNF-α (61-124BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-α.
To detect Murine TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine TNF-α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Reactivity:
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Mouse
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