Catalog Number : 500-P64G
Produced from sera of goats pre-immunized with highly pure recombinant Murine TNF-α. Anti-Murine TNF-α specific antibody was purified by affinity chromatography employing immobilized Murine TNF-α matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Murine TNF-α (BioGems Catalog #315-01A)
This antibody stained colchicine injected mouse brain (hippocampus CA1 region) tissue. The primary antibody was incubated at 1.0 μg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.
Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
To yield one-half maximal inhibition [ND50] of the biological activity of Murine TNF-α (0.25 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.
To detect Murine TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine TNF-α (61-124GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-α.
To detect Murine TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine TNF-α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.