Catalog Number : 500-P71
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF. Anti-Murine SCF specific antibody was purified by affinity chromatography employing immobilized mSCF matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Murine SCF (BioGems Catalog #250-03)
To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (20.0 ng/ml), a concentration of 0.64-0.97 µg/ml of this antibody is required.
To detect mSCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine SCF (61-119BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.