Catalog Number : 500-P215
Produced from sera of rabbits pre-immunized with highly pure recombinant Murine RELMβ. Anti-Murine RELMβ specific antibody was purified by affinity chromatography employing immobilized Murine RELMβ matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Murine RELMβ (BioGems Catalog #450-26B)
This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4°C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.
To detect Murine RELMβ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine RELMβ (61-116BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELMβ.
To detect Murine RELMβ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMβ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.