Catalog Number : 500-P129
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIP-10. Anti-Murine IP-10 specific antibody was purified by affinity chromatography employing immobilized mIP-10 matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Murine IP-10 (BioGems Catalog #250-16)
This antibody stained colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of mIP-10 (100.0 ng/ml), a concentration of 10.0 µg/ml of this antibody is required.
To detect mIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IP-10 (61-081BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.
To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.