Catalog Number : 500-P57
Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IL-7. Anti-Murine IL-7 specific antibody was purified by affinity chromatography employing immobilized Murine IL-7 matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Murine IL-7 (BioGems Catalog #217-17)
To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-7 (0.50 ng/ml), a concentration of 0.08-0.1 µg/ml of this antibody is required.
To detect Murine IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IL-7 (61-007BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-7.
To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.