Catalog Number : 500-P53
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-3. Anti-Murine IL-3 specific antibody was purified by affinity chromatography employing immobilized mIL-3 matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Murine IL-3 (BioGems Catalog #213-13)
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-3 (0.5 ng/ml), a concentration of 0.15 - 0.20 µg/ml of this antibody is required.
To detect mIL-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IL-3 (61-003BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-3.
To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.