Anti-Murine IL-2

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P111

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-2. Anti-Murine IL-2 specific antibody was purified by affinity chromatography employing immobilized mIL-2 matrix.

Additional Information

Application:ELISA, Western Blot, Neutralization
Immunogen
Anti-Murine IL-2 (BioGems Catalog #212-12)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (5.0 ng/ml), a concentration of 0.18-0.28 µg/ml of this antibody is required.
Sandwich ELISA:
To detect mIL-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IL-2 (61-002BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-2.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Mouse
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