Catalog Number : 500-P60
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-10. Anti-Murine IL-10 specific antibody was purified by affinity chromatography employing immobilized mIL-10 matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Murine IL-10 (BioGems Catalog #210-10)
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (1.0 ng/ml), a concentration of 0.043-0.064 µg/ml of this antibody is required.
To detect mIL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IL-10 (61-010BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-10.
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.