Catalog Number : 500-P51A
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1α. Anti-Murine IL-1α specific antibody was purified by affinity chromatography employing immobilized mIL-1α matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Murine IL-1α (BioGems Catalog #211-11A)
This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1α (0.05 ng/ml), a concentration of 0.0 3- 0.05 µg/ml of this antibody is required.
To detect mIL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IL-1α (61-001ABT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1α.
To detect mIL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.