Anti-Murine GM-CSF

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P65

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mGM-CSF. Anti-Murine GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix.

Additional Information

Application:ELISA, Western Blot, Neutralization
Immunogen
Anti-Murine GM-CSF (BioGems Catalog #315-03)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a concentration of 0.012-0.020 µg/ml of this antibody is required.
Sandwich ELISA:
To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine GM-CSF (61-075BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.
Western Blot:
To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Mouse
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