Catalog Number : 500-P67
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mEotaxin. Anti-Murine Eotaxin specific antibody was purified by affinity chromatography employing immobilized mEotaxin matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Murine Eotaxin (BioGems Catalog #250-01)
To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin (100.00 ng/ml), a concentration of 5.0-8.0 µg/ml of this antibody is required.
To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine Eotaxin (61-064BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin.
To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.