Catalog Number : 500-P251
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Anti-Human WNT-3a specific antibody was purified by affinity chromatography employing immobilized hWNT-3a matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/ml - 0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hWNT-3a by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human WNT-3a (60-403BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.