Catalog Number : 500-P222
Produced from sera of rabbits immunized with highly pure recombinant Human Visfatin. Anti-Human Visfatin specific antibody was purified by affinity chromatography employing an immobilized Human Visfatin matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human Visfatin (BioGems Catalog #130-09)
This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
To detect Human Visfatin by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Visfatin (60-347BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin.
To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.