Catalog Number : 500-P49G
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human TPO (BioGems Catalog #300-18)
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5.00 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.
To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TPO (60-329BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.