Anti-Human TECK

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P134

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK. Anti-Human TECK specific antibody was purified by affinity chromatography employing immobilized hTECK matrix.

Additional Information

Application:ELISA, Western Blot, Neutralization, Immunohistochemistry
Immunogen
Anti-Human TECK (BioGems Catalog #300-45)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel.  The recommended concentration is 0.5 μg/ml – 1.0μg/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval.  Optimal concentrations and conditions may vary.  
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/ml), a concentration of 10-15 µg/ml of this antibody is required.
Sandwich ELISA:
To detect hTECK by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TECK (60-305BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK.
Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Human
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