Catalog Number : 500-P134
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK. Anti-Human TECK specific antibody was purified by affinity chromatography employing immobilized hTECK matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human TECK (BioGems Catalog #300-45)
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 0.5 μg/ml – 1.0μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/ml), a concentration of 10-15 µg/ml of this antibody is required.
To detect hTECK by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TECK (60-305BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK.
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.