Catalog Number : 500-P135
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L specific antibody was purified by affinity chromatography employing immobilized hsTRAIL/Apo2L matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human sTRAIL/Apo2L (BioGems Catalog #310-04)
This antibody stained formalin-fixed, paraffin-embedded sections of human metastatic carcinoma of lymph node from breast tissue. The recommended concentration is 0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of Human sTRAIL/Apo2L (10.0 ng/ml), a concentration of 150 ng/ml of this antibody is required.
To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human sTRAIL/Apo2L (60-330BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTRAIL/Apo2L.
To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.