Catalog Number : 500-P48G
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hSCF. Anti-Human SCF specific antibody was purified by affinity chromatography employing immobilized hSCF matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human SCF (BioGems Catalog #300-07)
This antibody stained CACO-2 cells. The primary antibody was incubated at 2.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
To yield one-half maximal inhibition [ND50] of the biological activity of hSCF (10.00 ng/ml), a concentration of 0.008-0.012 µg/ml of this antibody is required.
To detect hSCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human SCF (60-291GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.